PHYTOCHEMICAL FINGERPRINT AND BIOLOGICAL ACTIVITIES OF THREE MALAYSIAN FICUS DELTOIDEA CULTIVARS.

Authors

  • Mohd Mukrish Mohd Hanafi School of Pharmacy, University College London, London, United Kingdom https://orcid.org/0000-0003-1259-0354
  • Omaish Salman O. Alqahtani School of Pharmacy, University College London, London, United Kingdom
  • Linna Gui School of Pharmacy, University College London, London, United Kingdom
  • Harisun Yaakob Institute of Bioproduct Development, Universiti Teknologi Malaysia, Malaysia https://orcid.org/0000-0001-6070-1990
  • Jose M Prieto School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom https://orcid.org/0000-0002-2649-1691

DOI:

https://doi.org/10.24377/jnpd.article668

Keywords:

Chemotaxonomy, Prostate Cancer, Tyrosinase, Antioxidant, Ficus

Abstract

Background

Ficus deltoidea (Moraceae), is a Malay Traditional Medicine locally known as Mas Cotek. Three varieties (angustifolia, deltoidea and kunslerii) has been indictincty used. 

Aims

We here aim to better understand their chemistry and bioactivities to inform future scientific and agronomic research.

Methods

We extracted and analyzed samples from these varieties by HPTLC and HPLC-UV. The in vitro screening looked up to the scavenging activities against both DPPH and NO radicals, inhibitory activity upon tyrosinase and cytotoxicity against human prostate cancer cells PC3, DU145 and LNCaP using the sulforhodamine B proliferation assay and the MTT mitochondrial viability assay.

Results

We achieved to analytically differentiate the three varieties  in term of the presence of flavonoids, establishing that vitexin, orientin and isoorientin may act as intraspecific and interorgan phytomarkers Their biological activities point out to the antioxidant value of extracts from the deltoidea and kunslerii varieties whilst the inhibition of tyrosinase is only present in the roots extract of the var. deltoidea which here we show is also endowed with cytotoxic activity against prostate cancer cells.

Conclusion

We suggest that the three Malaysian Ficus deltoidea botanical varieties (angustifolia, deltoidea and kunslerii) can be also considered chemovars. The least bioactive chemovar was angustifolia. The most active extract was from the roots of var. deltoidea that shows antioxidant, antimelanogenic and cytotoxic potential. 

Keywords: Chemotaxonomy; Prostate Cancer; Tyrosinase; Antioxidant; Ficus.

Author Biographies

Harisun Yaakob, Institute of Bioproduct Development, Universiti Teknologi Malaysia, Malaysia

Senior Lecturer

Jose M Prieto, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom

Associate professor in Natural Products (Phytochemsitry) (School of Pharmacy and Biomolecular Sciences)

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Published

2022-07-25

Issue

Vol. 1 No. 2 (2022): Journal of Natural Products Discovery

Section

Original Articles

License

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