IN VITRO EVALUATION OF THE ANTIMICROBIAL, TOXIC, ANTI-ARTHRITIC AND THROMBOLYTIC PROPERTIES OF DIFFERENT PARTS OF PLANT ORCHID RHYNCHOSTYLIS RETUSA (L.) BLUME.

Authors

  • Md. Saifur Rahaman Institute of Nuclear Science and Technology, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh.
  • Md. Samsur Rahaman Sonali Bag Project, Bangladesh Jute Mills Corporation, Ministry of Textiles and Jute, Bangladesh
  • SHAH MD. MARZUK HASNINE Institute of Nuclear Science and Technology, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh.
  • Salma Sultana Institute of Nuclear Science and Technology, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh.
  • Mohammad Shahriar Kabir Department of Chemistry, Primeasia University, Dhaka-1213, Bangladesh
  • Md. Abdul Bari Institute of Food and Radiation Biology, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh
  • Md. Ismail Hossain Agriculture Statistics Division, Bangladesh Rice Research Institute, Gazipur- 1701, Bangladesh
  • Jahid Md. Mahbub Islam School of Science, Monash University, Sunway Campus, Subang Jaya, Selangar 47500, Malaysia
  • Mubarak Ahmad Khan Sonali Bag Project, Bangladesh Jute Mills Corporation, Ministry of Textiles and Jute, Bangladesh
  • Md. Abdul Quaiyum Bhuiyan

DOI:

https://doi.org/10.24377/jnpd.article2348

Keywords:

Plant Orchid, Traditional Herbal Medicine, Antimicrobial Activities, Brine Shrimp Lethality Bioassay, Anti-arthritic, Thrombolytic, In Vitro Pharmacological Model

Abstract

Background: Infection due to microbes, trauma, cancer, arthritis and thrombosis are the most common problems worldwide. Traditionally, a large number of herbal extracts are used for the treatment of those disorders.

Aim: Ethanolic extracts from leaves, roots and stems of Rhynchostylis retusa (L.) Blume were phytochemically characterised and then tested for their antibacterial, toxicity, anti-arthritic, and thrombolytic properties in-vitro.

Methods and Results: Disk diffusion inhibition tests with a panel of fourteen different microorganisms was used as the antimicrobial assays. Antimicrobial activity of the stem extract found to be higher (10-11 mm, 400 µg/disk) than that of root extract whereas leaf showed no activity with ciprofloxacin (43-46 mm, 5 µg/disc) serving as reference standard. Toxicity of the plant extracts in terms of their LC50 values against the brine shrimp (A. salina) was assessed. The toxic potential of leaf extract was 6.7 μg/ml, which is higher than that of stem and root extract (c.a. 8.5 μg/ml). Thrombolytic properties were tested in vitro using streptokinase as reference (%Clot lysis after 1.5h & 24h was 76.15±1.94 & 92.59±2.35, 30000iu). The leaf extract exhibited higher activity. Leaf extract (1000 ppm) lysed of 33.08±1.24% of the clot and 60.47±2.33 after 1.5 h and 24 h of incubation, respectively. Anti-arthritic effects measured as albumin denaturation using diclofenac sodium as reference (IC50=60.25 ppm) revealed the stem extract as more active.

Conclusion: our research will help to provide evidence for some of the traditional uses of this medicinal plant.

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Published

2024-06-27

Issue

Vol. 3 No. 1 (2024): Journal of Natural Products Discovery

Section

Original Articles

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Copyright (c) 2024 Md. Saifur Rahaman, Md. Samsur Rahaman, SHAH MD. MARZUK HASNINE, Salma Sultana, Mohammad Shahriar Kabir, Md. Abdul Bari, Md. Ismail Hossain, Jahid Md. Mahbub Islam, Mubarak Ahmad Khan, Md. Abdul Quaiyum Bhuiyan

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