https://openjournals.ljmu.ac.uk/JNPD <p>JOURNAL OF NATURAL PRODUCTS DISCOVERY (JNPD) is devoted to the publication of original articles The Journal publishes papers describing significant novelty in the analysis, isolation of metabolites, biological and pharmacological&nbsp;properties of natural products (including whole plants, marine organisms, terrestrial animals, fungi, bacteria and viruses, their cells, tissues and organs. The derived extracts and isolates should have characteristics or activities relevant for either basic biological sciences (botany, ecology, biochemistry, pharmacology, etc.) or use in the food, agrochemical, pharmaceutical, cosmetic and related industries. All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products are also within the scope of the journal. Papers dealing with novel methods relating to areas such as data handling / data mining in plant sciences will also be welcomed.</p> Liverpool John Moores University en-US Journal of Natural Products Discovery 2755-1997 <p>This journal provides immediate open access to its content with no submission or publications fees. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a <a href="http://creativecommons.org/licenses/by/4.0" target="_blank" rel="noopener">&nbsp;Licence</a><a href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</a> that allows others to read, download, copy, distribute,&nbsp; print, search, or link to the full text of works in this journal. It also allows others to remix, adapt and build upon the work, as long as credit is given to the author(s).&nbsp;&nbsp;</p> https://openjournals.ljmu.ac.uk/JNPD/article/view/2579 <p><strong><em>Background: </em></strong>This study evaluates the antimicrobial activities, phytochemical composition, and thin layer chromatography (TLC) profile of ethanolic and aqueous leaf extracts of <em>Gossypium hirsutum</em> L. and <em>Abelmoschus esculentus (L.) Moench</em>.</p> <p><strong><em>Aims: </em></strong>The objective is to investigate the phytochemical constituents and assess the antimicrobial efficacy of these plant extracts against various pathogenic microorganisms.</p> <p><strong><em>Methods: </em></strong>Leaves were collected, identified, air-dried, and powdered. Ethanolic and aqueous extracts were prepared and subjected to TLC analysis using silica gel plates and a mobile phase of ethanol, methanol, and ethyl acetate (5:3:2). Phytochemical screening was conducted using standard protocols. Antimicrobial activity was evaluated using the agar well diffusion method against pathogens including <em>Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Proteus, Streptococcus pneumoniae</em> and <em>Candida spp</em>.</p> <p><strong><em>Results: </em></strong>TLC analysis revealed seven compounds in <em>G. hirsutum</em> and six in <em>Abelmoschus esculentus</em> with distinct Rf values. Phytochemical screening identified phenols/tannins, saponins, flavonoids, reducing sugars, terpenoids, and anthraquinones in <em>G. hirsutum</em>, while <em>A. esculentus</em> contained all tested phytochemicals except phytosterol and essential oil. The antimicrobial assay indicated that ethanolic extracts of both plants exhibited significant antibacterial activity, with <em>G. hirsutum</em> showing higher efficacy. Ethanolic extracts of <em>G. hirsutum</em> inhibited <em>S. aureus</em> and <em>S. typhi</em> effectively, while aqueous extracts showed limited activity. Extracts of <em>A. esculentus</em> leaves inhibited all tested organisms except Proteus, with ethanol extracts also ineffective against <em>S. pneumoniae</em>.</p> <p><strong><em>Conclusion: </em></strong>The study demonstrates that ethanolic extracts of <em>G. hirsutum</em> and <em>A. esculentus</em> possess antimicrobial properties. These findings support the potential use of these plant extracts in developing natural antimicrobial agents.</p> Chika Attama Luka Lawrence Olanipekun Gabriel Segun Copyright (c) 2024 Chika Attama, Luka Lawrence, Olanipekun Gabriel Segun https://creativecommons.org/licenses/by/4.0 2024-10-15 2024-10-15 3 2 10.24377/jnpd.article2579